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Could future iPhones come with a builtin panic button to discreetly summon the cops Its certainly a possibility, judging by Apples recent patent filings. The Epidemic Lp ZippysharePort St. Joe, Florida Wikipedia. St. Joseph Catholic Mission Church. Port St. Joe is a city located at the intersection of U. S. Highway 9. 8 and State Road 7. Gulf County, Florida, United States. As of the 2. 01. 0 census the population was 3,4. This was a decline from 3,6. Port St. Joe became the county seat of Gulf County in 1. HistoryeditSt. Joseph was founded in 1. Apalachicola, which was troubled by legal conflict over land titles. It was mostly abandoned in 1. In the early 2. 0th century a new settlement was founded close to the original after the arrival of the Apalachicola Northern Railroad in 1. It appears that the more informal Port St. Joe was adopted for official use around this time. GeographyeditPort St. Joe is located in southern Gulf County at 2. N8. 51. 75. 2W 2. N 8. 5. 2. 97. 78W 2. Florida Panhandle and along the Emerald Coast. According to the United States Census Bureau, the city has a total area of 1. The city limits extend into St. Joseph Bay, an arm of the Gulf of Mexico. U. S. Route 9. 8 passes through Port St. Joe as Monument Drive in the north and Constitution Drive in the south. US 9. 8 leads northwest along the Gulf shore 3. Panama City and southeast 2. Apalachicola. Florida State Road 7. Cecil G. Costin Sr. Boulevard leads northeast 2. Wewahitchka. Port St. Joe averages only 3 feet 0. At its highest point it is only 8 feet 2. This portion of the town has been dubbed Highland View. DemographicseditHistorical population. Census. Pop. 1. 93. Est. 2. 01. 63,4. U. S. Decennial Census9As of the census1. The population density was 1,0. There were 1,5. 71 housing units at an average density of 4. The racial makeup of the city was 6. White, 3. 0. 1. 0 African American, 0. Native American, 0. Asian, 0. 0. 5 from other races, and 0. Hispanic or Latino of any race were 0. There were 1,4. 02 households out of which 2. The average household size was 2. In the city, the population was spread out with 2. The median age was 4. For every 1. 00 females there were 8. For every 1. 00 females age 1. The median income for a household in the city was 3. Males had a median income of 3. The per capita income for the city was 1. About 1. 1. 2 of families and 1. Schools and librarieseditPort St. Joe has one elementary school, with Dolphins as mascot. It has a joint middle high school with Tiger Sharks as mascots. The school colors are purple and gold. There is also a small private school, Faith Christian School, which has the lions as its mascot. All of the public schools are located on one development strip set aside under the Department of Education. This begins with the elementary school and its playground and continues with the football stadium, a quarter mile running track, and finally the high school and adult school stretching a distance of about 5 city blocks. Port St. Joe also has a community college that offers two year associate degrees. Port St. Joe has a library co located with the senior citizens center, Sheriffs office, courthouse, and animal shelter off State Road 7. Port St. Joe almost became the capital of Florida in the 1. The state constitution was ratified in 1. There is a museum and monument commemorating this event. Local mediaeditWEBZ 9. FM UrbanW2. 16. BT 9. FM repeater of WFSW FM, Panama CityWDBW LP, 9. FM Long Avenue Baptist Church, repeater of Bible Broadcasting NetworkWOCY 1. FM Top 4. 0WOYS 1. FM Classic Hits. NewspapereditTransportationeditPort St. Joe is served by a variety of transportation modes. Port St. Joe is traversed by the Gulf Intracoastal Waterway GIWW, a federally maintained canal with a channel of 1. St. Marks, Florida, to Brownsville, Texas. From the GIWW, barges can be moved inland on various river systems including the Mississippi River, Tombigbee River Waterway System, and the Apalachicola River. The Apalachicola River is Gulf Countys eastern boundary. The Port of Port St. Joe shipping channel is congressionally authorized to a depth of 3. Gulf of Mexico and to the world. Existing facilities include a manufacturer of chemical lime from dolomite, a chemical plant, a 9 million tonyear dry bulk barge to rail transloading terminal, and a builder of dive cruise ships. The AN Railway serves the industries and port of Port St. Joe and has a history of transporting a variety of products including wood chips and other forest products, chemicals, and coal. In light of the latter, most of its 9. The AN connects with the Class I CSX Transportation at Chattahoochee, Florida. HighwayeditGulf Countys highway transportation needs are met with three main corridors U. S. Route 9. 8 along the coast and through Port St. Joe providing a route west to Panama City and east to Apalachicola, State Road 2. Wewahitchka to Panama City, and State Road 7. Port St. Joe northward toward Alabama and Georgia. Further, State Road 7. Interstate 1. 0, with only two traffic signals between Port St. Joe and Interstate 1. See alsoeditReferenceseditTransportation. Gulf County Economic Development Council, Inc. Retrieved on 2. 00. External linksedit. Ahead of Print Yellow Fever Virus in Urine and Semen of Convalescent Patient, Brazil Volume 2. Number 1January 2. Emerging Infectious Disease journal. Carla M. Barbosa. Nicholas Di Paola. Marielton P. Cunha. Mnica J. Rodrigues Jesus, Danielle B. Araujo, Vanessa B. Silveira, Fabyano B. Leal, Flvio S. Mesquita, Viviane F. Botosso, Paolo M. A. Zanotto, Edison L. Durigon, Marcos V. Silva, and Danielle B. L. Oliveira. Author affiliations University of So Paulo, So Paulo, Brazil C. M. Barbosa, N. Di Paolo, M. P. Cunha, M. J. Rodrigues Jesus, D. B. Araujo, V. B. Silveira, F. B. Leal, F. S. Mesquita, P. M. A. Zanotto, E. L. Durigon, D. B. L. Oliveira Butantan Institute, So Paulo V. F. Botosso Institute of Infectology Emilio Ribas, So Paulo M. V. Silva Pontifical Catholic University, So Paulo M. V. SilvaSuggested citation for this article. Abstract. Yellow fever virus RNA is usually detected in blood of infected humans. We detected virus RNA in urine and semen samples from a convalescent patient. A complete virus genome was sequenced for an isolate from a urine sample. This virus had a South American I genotype and unique synapomorphic changes. Yellow fever virus YFV is a member of the genus Flavivirus and causes yellow fever in humans, characterized by fever, prostration, and hepatic, renal and myocardial complications that lead to death in 2. Clinical confirmation of YFV infections is based on detection of virus RNA in blood by reverse transcription PCR or antigen based ELISAs. Detection of virus in urine samples has been used for confirming infections with flaviviruses, including West Nile virus 2, Zika virus 3 dengue virus 4, and YFV 5. Despite availability of an effective vaccine, 2. A large epidemic of yellow fever with high death rates recently occurred in Brazil. In December 2. 01. Minas Gerais cases were later identified in Esprito Santo, Gois, Mato Grosso, Par, Rio de Janeiro, So Paulo, Tocantins, and the Federal District. There were 7. 92 confirmed cases and 2. July 1. 0, 2. 01. We report a case of yellow fever in a 6. So Paulo and had not been vaccinated against yellow fever. The study protocol was approved by the Ethics Committee on Research with Human Beings at the University of Sao Paulo. The patient provided informed consent for use of the samples during the study. The patient had traveled to Januria, Minas Gerais, Brazil, on December 2. So Paulo on January 3, 2. On January 6, he had fever, chills, body pain, and nausea. Mirc Download Sites there. Program Mastering C Venugopal Pdf File here. During days 13 after symptom onset, more severe symptoms developed persistent fever temperature 3. C4. 0C, headache, body pain, prostration, vomiting, dizziness, anorexia, dark stools, dark yellow urine, and bitterness in the mouth. The patient was admitted to a public hospital in Januria on January 9. An ELISA for nonstructural protein 1 NS1 of dengue virus showed a negative result. The patient also had severe thrombocytopenia platelet count 7. Figure. Figure. Clinical progression and detection of YFV in urine and semen of convalescent patient, Brazil. A Platelet levels, clinical parameters and symptoms, and test results over a 1. On January 1. 3, the patient returned to So Paulo and was admitted to a public hospital. Another ELISA for dengue virus NS1 was performed and showed a negative result. His platelet count decreased to 5. On January 1. 6, the patient was admitted to a reference hospital for infectious diseases in So Paulo. He showed a moderate clinical presentation anicteric form and mild spontaneous hemorrhage ecchymosis in the right eye. High fever, gastrointestinal symptoms vomiting and diarrhea, weakness, adynamia, and generalized myalgia were also observed. The patient had a weight loss of 4 kg over 8 days. Serum and urine samples were obtained Figure, panel A Technical Appendix PDF 2. KB 3 pages. We extracted virus RNA by using the Nucli. SENS Easy. Mag Kit bio. Mrieux, Marcy ltoile, France. We tested samples for YFV by using a real time quantitiative reverse transcription PCR q. RT PCR and primers specific for YFV 8 and a conventional PCR and pan flavivirus primers 9. Serum samples showed negative results for both PCRs. However, a urine sample obtained 1. YFV cycle threshold Ct 1. RNA copiesm. L by q. RT PCR. We also performed a qualitative Ig. M capture ELISA with a specific virus antigen and obtained positive results optical density 1. On January 2. 7, we obtained serum, urine, and semen samples and tested them by using q. RT PCR. Urine Ct 2. RNA copiesm. L and semen Ct 3. RNA copiesm. L samples were positive for YFV. To evaluate infectivity, we tested a urine sample obtained on this date Technical Appendix PDF 2. KB 3 pages. We isolated YFV in cell culture, which confirmed virus integrity. We also confirmed infectivity after a second virus passage Ct 2. RNA copiesm. L. For the urine sample that was positive for YFV RNA by q. RT PCR, we directly characterized viral diversity by using next generation sequencing Technical Appendix PDF 2. KB 3 pages. In South America, phylogenetic studies have inferred 2 circulating YFV genotypes. The isolate from our patient BRMG 2. South America I isolates, including 2 viruses isolated in 2. Esprito Santo, a state bordering Minas Gerais, and other viruses isolated previously in Brazil Figure, panel B. We did not observe any insertions or deletions in BRMG 2. South America I strains. However, several synapomorphic changes were detected V1. I capsid, E1. 57. D NS3, R1. 60. 5K NS3, K2. R NS5, V2. 64. 5I NS5, G2. S NS5, N2. 80. 4S NS5, V3. A NS5, and N3. 21. S NS5. Most of these changes were located in the NS5 RNA dependent RNA polymerase gene, which plays a major role in virus replication. Changes in the NS5 gene have been associated with differences in viral replication, immune response, and proteinprotein interactions during virus replication. Our results suggest that semen can be a useful clinical material for diagnosis of yellow fever and indicate the need for testing urine and semen samples from patients with advanced disease. Such testing could improve diagnostics, reduce false negative results, and strengthen the reliability of epidemiologic data during ongoing and future outbreaks. Dr. Barbosa is a veterinarian and doctoral candidate at the University of So Paulo, So Paulo, Brazil, and Pirbright Institute, Pirbright, UK. Her research interests are molecular biology, cell culture, and animal and human virology. Acknowledgments. We thank Thaigo A. Souza and the technical team at Centro de Facilidades de Apoio a Pesquisa for providing assistance Cinthia Bombardieri and staff of the 2. PensabioIllumina Award Program for providing guidance and technical support and Luciano M. Thomazelli for providing technical support. This study was supported by Fundao de Amparo Pesquisa do Estado de So Paulo FAPESP projects 2. Conselho Nacional de Pesquisa CNPq project 4. N. D. was supported by a fellowship from FAPESP 2. M. P. C. was supported by a fellowship from FAPESP 2. M. J. R. J. was supported by a fellowship from FAPESP 2. V. B. S. was supported by a fellowship from FAPESP 2. C. M. B. was supported by a fellowship from CNPq 8. Coordenao de Aperfeioamento de Pessoal de Nvel SuperiorNewton Fund Institutional links 9. References. Beasley DW, Mc. Auley AJ, Bente DA. Yellow fever virus genetic and phenotypic diversity and implications for detection, prevention and therapy. Antiviral Res. 2.